It depends on the media you have loaded. What are you using? Images/Videos? In generaly, you can map just a single button. There are 3 play modes that you can select form though: Play Through (plays from the start to the end), Play While Held (plays while the sample trigger is held), and Play/Stop (trigger once to begin playback, and again to stop). Blend - selects the blending mode that is used to composite the sample (e.g. Multiply, Difference, etc). Taken from the manual, here: http://www.inklen.com/mixemergency/manual/sampleplayerwindow/